Design forward and reverse primers to the following DNA fragments. Primers are typically
∼20
nucleotides in length with annealing temperatures
∼50-60\deg C
, but only design these primers to be six nucleotides in length for simplicity, and write all primers
5^(')->3
'. Also calculate each primer's Tm and the annealing temperature that should be used in PCR.
5'-GTACTAGTAGACCGTATGCCGCAGTA-3'
Forward:
Reverse:
5!type your answer...
Tm:
type your answe
Tm:
type your answe
Annealing Temp:
type your answe
5'-GCTGTAAGAGGATITACTACCGTCCA-3'
Forward:
5type your answer...
Tm:
type your answe
Reverse:
5
Tm:
type your answe
Annealing Temp:
type your answe