Design forward and reverse primers to the following DNA fragments. Primers are typically 20 nucleotides in length with annealing temperatures 50-60 deg C, but only design these primers to be six nucleotides in length for simplicity, and write all primers 5^(')-3 '. Also calculate each primer's Tm and the annealing temperature that shoul

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Design forward and reverse primers to the following DNA fragments. Primers are typically

∼20

nucleotides in length with annealing temperatures

∼50-60\deg C

, but only design these primers to be six nucleotides in length for simplicity, and write all primers

5^(')->3

'. Also calculate each primer's Tm and the annealing temperature that should be used in PCR. 5'-GTACTAGTAGACCGTATGCCGCAGTA-3' Forward: Reverse: 5!type your answer... Tm: type your answe Tm: type your answe Annealing Temp: type your answe 5'-GCTGTAAGAGGATITACTACCGTCCA-3' Forward: 5type your answer... Tm: type your answe Reverse: 5 Tm: type your answe Annealing Temp: type your answe

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