Assist and help me with this...
1.Determine Vo by plotting the elution profile for Vo determination on graph paper
Figure 1: Absorbance reading of Dextran Blue elutions(600 nm)
|
(1ml/fraction) |
1 - 12 |
13 |
14 |
15 |
16 |
17 |
18 |
19 |
|
ABS |
0.000 |
0.014 |
0.485 |
0.534 |
0.283 |
0.088 |
0.049 |
0.000 |
2. What is void volume, Vo and what is been used to determine Vo in this experiment? explain your reasoning.